DOWNLOADS


DOCUMENTS

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USEFUL INFORMATION TO USE SEQUINS

Download useful documents including laboratory protocols, software user manuals, information on shipping and storing sequins, and white papers describing use and validation of sequins designs and concepts.

ID Description Download
P.R.10 Protocol for using RNA sequins in the laboratory. Includes information on dilution and addition to RNA samples. PDF
P.R.2 Anaquin software user guide. PDF
P.R.3 Material safety data sheet (MSDS) for RNA sequins. PDF
P.R.4 Shipping information for the sequins. PDF
P.R.5 Example customs declaration sheet that accompanies the sequins. PDF

MIXTURES

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CONCENTRATION OF SEQUINS IN EACH MIXTURE

Mixture file indicate the concentration of each sequin within a mixture (typically in attomoles/mL) and are often required to perform quantitative analysis. For each mixture we produce, we provide an associated mixture file that can be accessed below.

ID Description Download
M.R.12 Concentration of RnaQuin isoforms in Mixture A CSV
M.R.13 Concentration of RnaQuin isoforms in Mixture B CSV
M.R.14 Concentration of RnaQuin isoforms in Mixture A and B CSV
M.R.15 Concentration of RnaQuin genes in Mixture A CSV
M.R.16 Concentration of RnaQuin genes in Mixture B CSV
M.R.17 Concentration of RnaQuin genes in Mixture A and B CSV

ANNOTATIONS

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GENOME FEATURES ANNOTATIONS REPRESENTED WITH SEQUINS

Sequins represent genetic features, such as genes or variation, that are encoded in the in silico chromosome or genome. Annotation files indicate the location of such features and adhere to standard formats (such as .BED, .GTF and .VCF) wherever possible.

ID Description Download
A.R.1.2 Annotation for 78 synthetic gene loci comprising 164 isoforms GTF
A.R.2 Nucleotide sequence of RNA sequins FA
A.R.3 Kallisto index for RNA sequins Index
A.R.4 In silico chromosome (for RNA sequins) FA

LIBRARIES

icon_data_6EXAMPLE LIBRARIES FROM EACH SEQUIN MIXTURE

A range of simulated and experimental read libraries are available for download for example usage. These are to be used to complete tutorials and for users to familiarize themselves with the use and analysis of sequins.Additionally, we provide a neat sequenced library for each batch of sequins we release. Users may wish to check their sequencing and analysis relative to these reference libraries for troubleshooting or benchmarking purposes.

ID DESCRIPTION METADATA PAIR 1 PAIR 2
L.R.14 RNA sequins / Mixture A (pooled) .TXT .FQ .FQ
L.R.15 RNA sequins / Mixture B (pooled) .TXT .FQ .FQ
L.R.11 RNA sequins / Mixture B (replicate 1) .TXT .FQ .FQ
L.R.12 RNA sequins / Mixture B (replicate 1) .TXT .FQ .FQ
L.R.13 RNA sequins / Mixture B (replicate 1) .TXT .FQ .FQ

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